HR MAS

High Resolution Magic Angle Spinning (HR-MAS) is an established technique for analyzing intact biological tissue samples. By spinning at the magic angle, line broadening effects due to dipolar interactions and susceptibility differences within the sample are removed resulting in high resolution quality spectra.

HR-MAS probes are configured with a Z-gradient aligned along the magic angle enabling the researcher to access a wide variety of NMR experimental techniques including gradient enhanced solvent suppression and artifact free 2D homo-nuclear and hetero-nuclear experiments. Disposable rotor inserts are available for easy sample preparation and clean up. Here we present some practical recommendations for applying this technique to biological tissue samples.

Sample Analysis

NMR spectra are typically acquired at 4ºC to slow sample degradation and with a sample spin rate of 4 KHz.¹D¹H spectra with NOESY presaturation and/or¹D¹H CPMG filtered spectra are used for metabolite analysis via AMIX Software. 2D rotor synchronized adiabatic TOCSY and¹H/¹³C HSQC experiments aid in the identification of the metabolites of the samples.