NanoWizard 4 XP BioScience

The NanoWizard 4 XP BioScience comes with:

• PeakForce Tapping®for easy imaging
• Fast Scanning optionwith up to 150 lines/sec
• NestedScanner Technologyfor high-speed imaging of surface structures up to 16.5µm with outstanding resolution and stability
• New tiling functionalityfor automated mapping of large sample areas
• V7 Softwarewith revolutionary new workflow-based user interface
• DirectOverlay™ 2 softwarefor perfect integration and data correlation with advanced fluorescence microscopy platforms
• Vortis™ 2 controller高速信号处理和最低噪音levels

The HybridStage™ or Motorized Precision Stagetransforms experiments by enabling direct access to a large sample area, with automated, motorized movement to selected positions, grids and mapping regions.

Begin with the DirectOverlay 2 optical calibration, and then select a region for optical tiling up to millimeters in size.

精确的motor movements automatically bring the whole sample into view, making it easy to select regions and features for further investigation. A single click navigates from point to point or MultiScan experiments automate a sequence of measurements at selected points.

The images show living Vero cells in cell culture medium at 37°C in the PetriDishHeater™ . [2] - [5] Optical tiling with 5×6 phase contrast images covering a 630µm×450µm region. - Zoom into region scanned with AFM showing 100μm×100μm scan (height range 5μm) and inset 15μm×15μm (height range 2μm) scan topography images using PeakForce Tapping. The feedback correction signal images highlight the surface membrane features, particularly in the zoomed image. Microvilli dominate the center of the cell, with membrane ruffles at the cell boundary.

The images show NanoWizard 4 XP setups on Zeiss LSM 880 confocal microscope with Airyscan [1] , with Upright Fluorescence Microscope (UFM) Kit for tissues or other large samples such as organs [2] , with BioMAT Workstation for high NA optics and Zeiss Axio Imager [3] , with TopviewOptics [4] and on on Olympus with PicoQuant MicroTime 200 STED [5].

QI™ Advanced, based on real force curves, offers both astounding speed and resolution for applications ranging from single molecules to living cells. The quantitative data allows precise and fast analysis of mechanical or biochemical interactions, e.g., localization of binding sites, directly overlaid with fluorescent labelling and topography with Molecular Recognition Imaging. Advanced batch processing options include multiple models for modulus fitting and can reveal surface topography at zero force with Contact Point Imaging (CPI).

The image shows single cell force spectroscopy measurements using the CellHesion module with an increased z range of 100 µm, showing the detachment force curves of a single A549 cell from fibronectin (FN) and from bovine serum albumin (BSA) coated culture dishes. Note, that the detachment of the cell from fibronectin results in a very large pulling range of 77 µm.

• Fast Scanning optionwith up to 150 lines/sec for tracking dynamic processes
• Now with Bruker’s exclusive PeakForce Tapping as standard
• Large scan field of 100 × 100 × 15 µm³and atomic lattice resolution on inverted microscopes
• New workflow-based user interfacefor ergonomics and ease of operation
• New tiling functionalityfor automated mapping of large sample areas together with the HybridStage
• Enhanced DirectOverlay 2 mode for most precise correlative microscopy
• New Vortis 2 controllerwith high-speed low-noise DACs and cutting-edge position sensor readout technology
• Highest flexibility and upgradeabilitywith a broad range of modes and accessories
  

Standard Operating Modes

Imaging modes

• Now with PeakForce Tapping
• Contact mode with lateral force microscopy (LFM)
• Tapping Mode™ with PhaseImaging™

Force measurements

• Static and dynamic spectroscopy
• Advanced force mapping

• Higher harmonics imaging
• Kelvin Probe Microscopy and SCM MFM and EFM (see also QI mode)
• Conductive AFM (see also QI mode)
• STM
• Electrical spectroscopy modes
• Piezoresponse Microscopy for high voltages
• Electrochemistry with temperature control and optical microscopy
• NanoLithography
• NanoManipulation
• Nanoindentation
• Scanning Thermal AFM
• FluidFM® solution from Cytosurge
• ExperimentPlanner for designing a specific measurement workflow
• RampDesigner™ for custom designed clamp and ramp experiments
• ExperimentControl feature for remote experiment control
• DirectOverlay 2 for combined AFM and optical microscopy
• Additional XY or Z sample movement stages available with CellHesion®, TAO™ and HybridStage™ module
  

Optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions and more. Bruker provides you with the right accessories to control your sample conditions and to perform successful experiments.

The new V7 software interface guides users through the workflow to set up experiments intuitively and makes it simple, even for users with minimal AFM experience, to progress confidently to generating high-quality data. Each stage of the setup and operation works as an optimized desktop that brings all the vital information into focus with a single click.

• On-screen, context-sensitive help
  
• Status feedback for alignment and setup
• Efficient task-based experiment selection
• Fast access to favorite and recently used experiments
• One-click probe calibration
• Instant overview of the key data
• User management for Multi-User environments like imaging facilities