MALDI-TOF/TOF

The analytical advantages of matrix assisted laser desorption ionization (MALDI) TOF MS have been demonstrated for more than 25 years. With inherent benefits including its speed (time to results) and application flexibility, the convenience and effectiveness of MALDI-TOF MS is magnified by virtue of its low sample volume requirements and broad sample content (salts, buffers) amenability. Dramatic increases in the depth of MALDI analyses have been made routine by intuitive software, and easy automation allows for high throughput solutions.

Bruker’s FLEX series instruments provide reliable results from a market leading technology platform - well-known for outstanding performance, reliability, convenience and innovative design. From rapid screening to comprehensive target characterization, MALDI-TOF MS offers broad utility to meet all laboratory needs. Expert users and beginners can quickly and confidently tap into the analytical power and flexibility of Bruker’s FLEX series MALDI-TOF systems.

Over the last 25 years, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry has evolved into a powerful, highly versatile technology with a diverse range of applications. MALDI coupled to time-of-flight mass spectrometry (MALDI-TOF MS) is used to sequence proteins, map biomolecules in tissues, identify microorganisms, and analyze several thousand biochemical assays in a day.

The sample for analysis by MALDI-TOF MS is prepared by mixing or coating with a solution of an energy-absorbent matrix which entraps and co-crystallizes the sample when dried. The matrix is ionized with a laser beam, and transfers the charge to the analytes, generating singly charged ions from analytes in the sample that are then accelerated. Ions are separated from each other on the basis of their mass-to-charge ratio (m/z) before being detected and measured using the TOF mass analyzer.

The high throughput and speed associated with MALDI-TOF MS has made this technology an important tool for large-scale proteomics, imaging studies, and other analyses.

Since the discovery of MALDI in the mid 80's this fast and high-throughput enabling ionization made its way into a variety of applications. The inherent speed of MALDI-TOF analysis from sample to result allows for fast answers to your analytical questions. The laser aiming at different positions gives rise to spatial, molecular information as used e.g. for MALDI Imaging for tissues.