Metabonomics to the next level: by nuclear magnetic resonance (NMR) to strengthen international cooperation

Want to get from human disease metabonomics research statistical significance, requires a large group of patients with willing and able to participate in medical research, and one can deal with small sample and provide quantitative and repeatable analyte concentration of the instrument.Dr Christoph at the university of tubingen in Germany Trautwein was able to solve the two problems, thanks to the power of nuclear magnetic resonance (NMR).Dr Brooke and Trautwein together thoroughly discusses his research work.

Dr Christoph Trautwein's research covers the preclinical and clinical research, as well as a wide range of medical scenarios - from neurodegenerative diseases, such as Parkinson's and alzheimer's disease, and breast cancer, hepatocellular carcinoma and glioma, stroke, and inflammatory bowel disease.

Identify with the new crown pneumonia (Covid - 19) metabolites and lipoprotein

But since the early 2020 Covid - 19 pandemic began, his research focus is increased.Dr Trautwein said: "we just want to make some contribution to the research of Covid - 19.Initially, this work involves the tubingen university medical genetics department colleagues, we investigated and there 'cytokine storm (cytokine storm)' on immune cells, which is a major reason that resulted in the deaths of Covid - 19¹.

"The first part is the basic research of metabolites," Dr Trautwein said: "the use of nuclear magnetic resonance (NMR), we found that the metabolism of active infection of the patients had obvious characteristics, in accordance with the existing research.Then, with the help of brooke provides a software package, they began to do some quantitative work."He added: "we found that there are several kinds of metabolites in plasma content is higher, it is very interesting, because these metabolites are usually associated with insulin resistance, cardiovascular disease and inflammation."

Work in progress: delve into new crown pneumonia symptoms (Long - Covid) for a Long time

Dr Trautwein said the finding may study has important significance for Long - Covid: "because I used in neuroscience research², I would like to survey Covid - 19 very interested in the impact of the brain.Maybe we find these metabolites in, there may be a kind of metabolite can tell us why some people with Long - Covid appeared headache or memory and attention problems."

Now, Dr Trautwein has started with the university of Heidelberg, Germany on this subject has carried on the new cooperation, the project will use the nearly 350 people in serum and urine samples, these are the new crown pneumonia virus (SARS - CoV - 2) in severe cases, some of them also had Long - Covid.He said: "this work is under way, but we have to get the participants of the original data, we are looking forward to find some interesting insights."

Convenient and high sensitivity analysis) with the nuclear magnetic resonance (NMR)

But he also added that the opportunity is accompanied by the challenge of - in this case, it is just difficult to accurately see brain internal events.He believes in this regard, magnetic resonance imaging (MRI) is a good tool, but expensive, and display only primary brain injury: "you can't do every week!"But based on nuclear magnetic resonance (NMR) metabolomics is cheaper, easier, "as long as you have installed hardware, in principle, every day you can measure a sample".The nuclear magnetic resonance (NMR) data for use as a research with nervous system symptoms of Covid - 19 patients with a more convenient way to provide the possibility, first of all the data associated with MRI scans.

Trautwein, says Dr Brooke 600 MHz of tubingen NMR Avance IVDr instrument in his a lot of research has played a central role, because it is for quantitative screening high-throughput biological liquid tailored: "have a high reproducibility and high sensitivity of the system is very important to test our sample in a small number of metabolites, and because it can hold 3 mm tube, rather than the conventional type of 5 mm, we only use 100 (including L scarce patient samples (can) screening."

, said Dr Trautwein run on Avance IVDr experiment has a unique advantage: "all Avance IVDr system have a very helpful aspects, that is they use the same hardware, software, and standard operating procedures, which means that the data obtained in different laboratories can compare immediately."

Promote cooperation effect of comparable data

, explained Dr Trautwein cross the comparability of results and highly relevant Covid - 19 research: "Covid - 19 is a challenge, and relatively small number of patients are willing or able to join the research project, this means that a lot of research is very difficult to achieve statistical significance."

But data set from the Avance IVDr nuclear magnetic resonance (NMR) system of comparability means Trautwein Dr And his colleagues can collect data set: "therefore, we are from Australia, Spain, Germany, Britain and Italy samples has carried on the comparative study, this allows us to filter out confounding factors, and a better understanding of Covid - 19 in the acute and long-term effects."

As a result, he said, due to the brook of nuclear magnetic resonance instrument strong analysis ability, metabonomics has reached a new level: "when you're dealing with complex diseases, whether Covid - 19, cancer, neurodegenerative diseases, or metabolic disease, you can with the experts from all over the world to share data and professional knowledge, enable us to more progress faster.This is the way of science should really works!"

References:

1. Singh Y., c. Trautwein et al., 2 infection SARS - CoV - paralyzes cytotoxic and metabolic functions provides of the immune cells, Heliyon, 2021 (6) : 7 e07147,https://doi.org/10.1016/j.heliyon.2021.e07147
2. C. Trautwein et al., Tissue metabolites in diffuse glioma and their modulations by IDH1 mutation, histology and treatment, JCI Insight, 2021,https://doi.org/10.1172/jci.insight.153526
3. Lucia Laugwitz, Laimdota Zizmare, Vidiyaah Santhanakumaran, Claire Cannet, Judith Bohringer, Jurgen g. Okun, Manfred Spraul, Ingeborg Krageloh - Mann, Samuel Groeschel, Christoph Trautwein, Identification of neurodegeneration indicators and diseases progression in metachromatic leukodystrophy using policy NMR - -based urinary metabolomics, JIMD reports, 2022,https://onlinelibrary.wiley.com/doi/10.1002/jmd2.12273

Dr Christoph Trautwein in studying environmental engineering, university of Stuttgart, Germany, and then in 2007 started a second job at the university of freiburg, molecular medicine, and completed the doctorate.In the finish after a postdoctoral fellow at karlsruhe institute of technology, in 2017, he joined the university of tubingen in Werner - Siemens imaging center, at present, where he led the metabonomics and the system of medical research group.

Brooke is committed to supporting scientists acquire the groundbreaking scientific discoveries and new applications are developed to improve the quality of life of human beings.Brooke's science and technology of high performance instruments and high value analysis and diagnosis of solution, allow scientists to the molecules, cells and micro level to explore the mysteries of life and material.In close cooperation and user, brooke devotes to the scientific and technological innovation, improve productivity and achieve the success of the user.Our business areas include molecular life science research, and drug application, microscope and nanometer analysis, industrial application, cell biology, preclinical imaging, clinical phenotype omics, proteomics research and clinical microbiology and so on.