OligoQuest ™

Nucleic acid for research, diagnosis, and gene therapy (including tRNA, siRNA and other modified nucleic acid) as many as 120 bases of the importance of increasing, regulators also promoted the sequence validation, identification and quantitative awareness and related impurities.RNA and accuracy of oligonucleotide sequence, to ensure that the correct activity, expression and avoid off-target effects or toxicity of patients is very important.However, the second generation sequencing methods cannot be comprehensive characterization of highly modified RNA drugs.

Currently using quadrupole time-of-flight mass spectrometry (QTOF - MS) instruments characterization of highly modified nucleic acid sequence, because they were able to high quality precision, determine the complete nucleic acid and single isotope mass fragment ions.

Impurities and by-products of nucleic acid molecular weight complete confirmation is not always can meet all the requirements, so you need to the level of nucleotide sequence information.For longer RNA molecules, need combined with MS/MS experiment and digestion, to complete identification of the LC - MS peak.Because the nucleic acid MS/MS spectra is relatively complex, artificial parsing is very time consuming, and requires skilled analytical skills, make it difficult to analyze multiple samples in a day, expensive and often into the bottleneck.

Brooke OligoQuest ™ software for the study of the antisense, RNA drugs, the CRISPR wizard confirmation RNA sequences, as well as other gene editing technology, provide more excellent RNA oligonucleotides and characterization.Brooke maXis II and timsTOF platform high fidelity of isotope and quality precision, OligoQuest ™ can complete molecular weight and MS2 analysis, qualitative RNA oligonucleotides and confirmation.Many customers are using OligoQuest ™, to confirm the isomer of oligonucleotides modified RNA sequences and cation exchange.

OligoQuest ™ provides the algorithm and workflow, to comment more than more than 100 tandem MS data of nucleotide sequences of nucleic acids.RiboDynamics chief executive and the university of Connecticut, a senior professor Dan Fabris explained: "the fidelity of isotope and the combination of high resolution mass spectrometry in analysis of highly modified RNA has great prospects, but there has been no applicable commercial software tools.Using OligoQuest ™ to simplify the analysis, will greatly speed up research and development in the field of RNA."

BioPharma Compass^®The OligoQuest ™ workflow is the wizard type drive, this simplified method Settings, and to meet customer demands for nucleic acid sequence of the custom, various modified nucleotide is widely used include the pharmaceutical industry.

OligoQuest ™ with flexible sequence editor, support natural nucleic acid structure unit, and custom skeleton, bases, and sugar.Using the algorithm of the well-tested, with high fidelity of isotope and ultra-high resolution to peak detection of MS and MS/MS data reconciliation convolution, even high dynamic range of the samples, also can ensure correct quality distribution.

TimsTOF series mass spectrometer or maXis II VIP - HESI ion source, has a high sensitivity in the negative ion mode, is oligonucleotide (such as siRNA), and larger molecules (such as CRISPR editor of single wizard RNA gene (sgRNA) choice of heterogeneity analysis.

Real isotopic pattern and the SNAP algorithm can obtain accurate quality distribution in the complex spectra, even for only one Da base substitution of quality differences, also can achieve reliable sequence verification.

Reliable to complex overlapping spectra of the annotations, is vital for the sequence confirmed with MS/MS.OligoQuest ™结合了 SNAP 解卷积和 RiboDynamics 的高级测序算法 ( 内置于 BioPharma Compass^®), making full use of the maXis II and timsTOF good data quality to comment and internal segments.

Nucleic acid by-product may contain random sequence variants, and cytidine and uridine transformation, respectively with the target sequence of product molecular weight + 1 Da.Only they were characterized according to the molecular weight is very difficult to complete, and therefore need nucleotide level information.

Nucleic acid MS/MS spectra is relatively complex, its artificial parsing is very time-consuming, and require skilled analytical skills, make routine laboratory comprehensive analysis of multiple samples in a day are difficult and expensive.

BioPharma Compass^®The OligoQuest ™ workflow for parsing the nucleic acid MS and MS/MS spectra

Provides a tool of easy to learn.Brooke's high resolution (HRAM) QTOF and precise quality timsTOF instrument with high fidelity of isotope, and mature SNAP for single isotope peak recognition algorithm, and can provide reliable for quality of mother ion and fragment ions.Therefore, MS and MS/MS spectra can be as the target sequence and expected by-products such as height matching.OligoQuest ™具有用户自定义序列设置，从而将实验室特定的序列轻松转换为软件支持的格式。结合 BioPharma Compass^®Proved in the workflow wizard, easy, and report the result of the process, OligoQuest ™ helps speed up the characterization of DNA or RNA, and meet flux needed for the pharmaceutical industry.

Network seminar held on April 5, 2022

OligoQuest ™ : use LC - MS/MS) the oligonucleotide new characterization workflow

As DNA and RNA as research reagents as well as the use of the drug molecules is becoming more and more mass spectrometry for the oligonucleotide characterization attracted much attention in recent years.In this kind of biological pharmaceutical product approval process, related characterization of impurities or degradation products is vital.Used for the characterization of the process of mature tools are based on the method of LC - MS.During the lab meeting live, we will showing how brooke high-resolution mass spectrometer is suitable for the analysis of oligonucleotides.We will demonstrate new software developed by OligoQuest ™, it can simplify the characterization of oligonucleotides and their degradation products.

OligoQuest related information