JPK ForceRobot biological type atomic force microscope
Bright spot
ForceRobot 400
Force plays an important role in molecular mechanism, such as identification, response and signal transmission.ForceRobot ® 400 biological AFM is a combination of many unique force spectrum innovative technology to measure the force at the single molecule level.It is able to quantify the mechanical strength of a single molecular bonds, and the molecular interactions and biological molecular force depend on the characteristics of the composites were characterized.
The characteristics of
Understanding single molecule mechanics
ForceRobot 400 has a unique ability to provide key insights for functional biological mechanisms, biophysics, biochemistry, developmental biology and new therapeutic molecules researches, such as the development of new possibilities.
ForceRobot 400 provides:
Excellent performance: every 250000 force curve
Fully automated single molecular force spectrum measurement
Biomedical and clinical research before need statistical correlation, standardization of data sets
Advanced force curve design and the wider fitting procedure, realize flexible experimental design
Original parameter nano force spectrum
ForceRobot 400 under near physiological conditions for a single molecule marker of nano mechanical measurement.Can collect high quality related data sets, multiple parameters, and obtained automatically from each data set comprehensive biophysical parameters.
"Raising the level of automation, combined with the advanced optical technology, makes the new ForceRobot 400 on single molecule scale study biophysical phenomena of outstanding tools.This load capacity enables people to study at the single molecule level on the mechanical properties of the natural and synthetic polymers, promote the design and synthesis of new macro and nano materials."
- from the Chinese department of physics of nanjing university professor cao
Advanced automation power spectrum measurement
, 400 is the ideal tool to SMFS ForceRobot for molecular biophysics mechanism provides key insights.Large size samples of Z axis motor and enhanced electric machine function makes it able to from the single molecule to the larger organic compounds, and are far apart or in different samples of interested area (ROI) were studied.The latest optional NestedScanner has a rapid Z axis scanner with capacitive sensors.It even under high speed could generate reproducible force curve, greatly expanded the micro rheological measurement frequency range.
New SmartMapping allows flexible two-dimensional tries to select multiple, user defined.Using optical tiling, can choose multiple ROI and automatic test in advance, to facilitate the system study on the large sample area.
Set the new standard of single molecular force spectrum test automation
ForceRobot 400 have fast rate of data acquisition, advanced automation and analysis ability.Combining with proprietary software, autofocus and calibration function ensures that the automation and rapid results.
The flexibility of acme
The new ForceRobot 400 user interface, with intuitive user guide, able to quickly define operating parameters.Predefined experimental setup, such as power tongs, force or micro rheology, combined with the easy-to-use editing tools, allows the user to define the experimental design.Thanks to automatically adjust the system parameters, can be long-term, series of experiments.
Breakthrough ability
An hour more than 10000 force curve
Af detection system and adaptive environmental conditions
Detection of rough surface of new type automatic adjust imaging mode
Modulation frequency up to at least 5 KHZ viscoelastic property characterization (optional Z axis scanning tube)
A wide range of environmental control accessories (humidity, temperature, ionic strength, buffer exchange, etc.)
Seamless combination with optical microscope
ForceRobot 400 can with advanced optical and super-resolution seamless integration technology, provide relevant nano mechanics data set, and the comprehensive characterization on the various kinds of biological samples.
ForceRobot 400 on Nikon Eclipse Ti2 inverted microscope and intuitive software interface.
In nikon Eclipse Ti2 ForceRobot 400 on the inverted microscope and intuitive software interface.
application
Can use ForceRobot 400 study:
- Molecular protein function
- Molecular biomechanics and key receptor and the ligand types
- For a new type of antibody immune diagnosis - antigen complex of detection and kinetics studies
- The viscoelasticity of different material
- Maximum adhesion force, a single combination and the mechanism of the combination of events
- Within the molecular characteristics of single molecules and molecular arrangement
- Macromolecular structure change
Using ForceRobot technology selection of academic papers published
- Ms Fernandez et al., AFM - -based Force Spectroscopy Unravels Stepwise Formation of the DNA Transposition Complex in the Widespread Tn3 Family Mobile Based Elements. Nucleic Acids Research, gkad241 (2023).
- Dupuy et al., 雷竞技怎么下载Molecular Device for the Redox Quality Control of GroEL/ES Substrates. Cell, 186 (5), 1039-1049. The e17. (2023).
- Li et al., the Active Microrheology of Protein Condensates Using Colloidal Probe - AFM. Journal of Colloid and Interface Science, 632, 357-366 (2023).
- Blaimschein et al., Substrate - Binding Guides Individual Melibiose Permeases MelB to Structurally Soften and Destabilize Cytoplasmic Middle - Loop C3. 31 (1) Structure, 58-67. The e4 (2023).
- Serdiuk et al., A Cholesterol Analog Stabilizes the Human beta 2 - Adrenergic Receptor Nonlinearly with Temperature. Sci. Signal. 15 (737), eabi7031 (2022).
- Petitjean et al., 9 - O - Acetylated - Multivalent Sialic Acid Glycoclusters as Potent Inhibitors for SARS - CoV - 2 Infection. Nat Commun 13 (1), 2564 (2022).
- Lee, et al., Modified Cytosines versus Cytosine in a DNA Polymerase: Retrieving Thermodynamic and Kinetic Constants at the Single Molecule Level. The Analyst, 147 (2), 341-348 (2022).
- Lei et al., the An Ester Bond Underlies the Mechanical Strength of a Pathogen Surface Protein. The Nat Commun 12 (1), 5082 (2021).
- Wang et al., Living 雷竞技网页版Materials Fabricated via Gradient Mineralization of Light - Inducible Biofilms. Nat Chem Biol 17 (3), 351-359 (2021).
- Liu, et al, High Force Catch Bond Mechanism of Bacterial Adhesion in the Human Gut. Nat Commun 11 (1), 4321 (2020).
- Zhang et al., the Dynamic Topology of Double - Stranded Telomeric DNA is Studied by Single - Molecule Manipulation in Vitro. Nucleic Acids Research, 48 (12), 6458-6470 (2020).
- Sun et al., 雷竞技怎么下载Molecular Engineering of Metal Coordination Interactions for Strong, Tough, and Fast - Recovery Hydrogels. Sci. Adv. 6 (16), eaaz9531 (2020).
- Huang et al., Maleimide - Thiol Adducts Stabilized through Stretching. Nat. Chem., 11 (4), 310-319 (2019).
- Tiu system et al., Enhanced Adhesion and Cohesion of Bioinspired Dry/Wet Pressure - Sensitive Adhesives. The ACS Appl. Mater. Interfaces 11 (31), 28296-28306 (2019).
ForceRobot 400 data to demonstrate
Bruker's BioAFMs allow life science and biophysics researchers to further their investigations in the fields of cell mechanics and adhesion, mechanobiology, cell - cell and the cell - surface interactions, cell dynamics, and cell morphology, We have collected a gallery of images demonstrating what of these applications.
accessories
One of the most abundant accessories on the market
Optical system/accessories, electrochemical solution, electronic sample characterization, environmental control options, software module, temperature control, acoustics and vibration isolation solution and so on.Brooke company to provide you with the appropriate accessories, to control your samples conditions and to carry out the successful experiment.
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