EPR spectrometer

Electron paramagnetic resonance (EPR) technique in the field of industrial applications

In the industrial environment, we can use electron paramagnetic resonance (EPR) analyzing various properties of products: food and beverage oxidation stability and oxidation resistance, radiation dose, for sure, for the product after irradiation, polymer analysis to determine failure rate, and the diamond quality.

The oxidation stability of food and drink

The oxidation stability of olive oil EPR quantitative analysis

Oxidation stability is a major problem facing the food industry, it is affected by many factors, such as oxygen, temperature, and light metal.With extra virgin olive oil (EVOO), for example, because of its distribution channels in all over the world is very complex, and it is a kind of independent packing product (the final quality will be a positive/negative impact on producers), so the oxidation of special attention.Extra virgin olive oil oxidation resistance and a high level of monounsaturated triglycerides and natural phenolic antioxidants.EPR is the detection of free radicals and determination of olive oil in forced oxidation under different temperature levels of free radicals in the process of useful tools.In the food industry using the method of EPR, can reveal is closely related to food quality and metamorphism of free radical reaction of important information.

Under 40 ℃, the spin trapping agent is added to the olive oil samples (DMPO), detect the EPR spectra.Respectively in the figure shows the simulation experiment spectra and three kinds of free radicals of simulated spectra.
In the process of the oxidation of 40 ° C, olive oil of hydrogen peroxide, alkoxy, concentration of alkyl DMPO radical adduct and DMPO form of oxidation

Antioxidant capacity

Removed by DPPH measurement to measure the antioxidant activity of drinks

Antioxidants play important roles as health protection factor.Have scientific evidence suggests that antioxidants can reduce your risk of chronic diseases, including cancer and heart disease.DPPH (2, 2 - Diphenyl - 1 - picrylhydrazyl) is a kind of free radicals, is widely used in the test of compound ability as a free radical scavenger or hydrogen donor and evaluation of antioxidant activity.DPPH method is based on the reduction of DPPH, antioxidants measured antioxidant capacity in food and beverage is a simple and rapid method.

By DPPH QingChuFa determination of antioxidant activity of wine and tea samples

Radiation dosimetry

Through the EPR spectrum method to detect alanine free radicals and irradiation dose

Alanine when exposure to ionizing radiation, can form very stable free radical.Alanine EPR signal from the free radicals related to dose, but has nothing to do with the dose rate, energy type, and is not sensitive to temperature and humidity relatively.Therefore, alanine dosimetry applies to gamma, electron beam or X-ray irradiation facilities.

Alanine the EPR signal and the reference mark of free radicals

Pharmaceutical analysis

Lactose free radicals of tablet filler will increase the degradation of active pharmaceutical ingredients (API)

EPR spectrum method is widely applied in the analysis of drug compounds, including API light degradation and oxidation, the effect of irradiation sterilization technology such as, the interaction between the API and excipient.Excipients can trigger, transmission, or participate in free radical chemistry interaction, which may damage the effectiveness of the drug, can through the EPR spectrum method about this study.From lactose monohydrate lactose tablets of free radicals is used as a filling agent, it will react with API, adding to the degradation of active pharmaceutical ingredients.

Food science and drinks

After ionizing radiation of poultry and fruit can produce a very unique EPR spectra

Food irradiation is used to reduce health risks associated with foodborne pathogens and prolong shelf life.In fact, ionizing radiation can inhibit microbial divided, radiation decomposition product and the free radicals.In the dry environment, these free radicals is very stable.After irradiation of poultry bones or fruit, for example, may contain a large number of stable free radical, these free radicals can easily detected by the EPR spectrum method.

After irradiation treatment of chicken bone EPR spectra
After irradiation treatment of mango EPR spectra

Polymer research

Through the EPR spectrum method detected polyethylene free radicals to predict premature failure of the implants

In the field of orthopaedic implants, ultra-high molecular weight polyethylene (UHMWPE) is used as a standard lining material.Oxidative degradation caused by free radicals to form polymers, can lead to material and implant premature aging and wear and tear, causing the inflammatory pain.The EMXnano polyethylene can detect and quantify free radicals, thus providing accurate and reliable measurement.

Two different kinds of polyethylene the EPR spectra of free radicals
This data by Cambridge Polymer Group Gavin Braithwaite, Dr

The diamond quality assessment

Through the EPR spectrum method detect N3 and replace nitrogen center in diamond

EPR spectrum method is one of the very clear nitrogen center quantitative analysis technology, thus provides a color classification tool.In addition, the EPR spectrum method can also be used to distinguish between synthetic diamond and natural diamond.

At room temperature, different grades (color) diamond EPR spectra