Non-uniform sampling (NUS)

Non-uniform sampling (NUS) is a kind of used in NMR experiment in which two or more dimensions of acquisition method.Usually take it skipped the random part of the data, thus saving the sampling time.Reconstruction algorithm is used to get the right from the sparse data set spectrum.

NUS multidimensional experiments of biomolecules nuclear magnetic resonance (NMR) is particularly useful, in the 4 d and 5 d experiments can save up to 10 times or more of the time.With NUS, the new used in the determination of protein structure becomes possible.

The small molecules based on 2 d spectrum application can also use acceleration sampling.NUS without any increase in the overall acquisition time improves the resolution spectra and quality, this is the main reason for using it.

Non-uniform sampling (NUS) has been fully integrated into the collection and processing of TopSpin.It support the routine use, can be used for all types of NMR experiment.It is a kind of automatic generation and optimization of NUS list defined by only a small amount of data acquisition sampling mode.Then, multidimensional decomposition allows the missing data points, with complete data set of the conventional Fourier transform processing, all of these are finished in fully automated way.

NUS experimental setup and perform contained in the License of TopSpin, also including the processing of 2 d spectra.Dealing with 3 d - 5 d NUS spectrum requires additional License.

NUS application in TopSpin is with Orekhov professors and the research results of the cooperation of the colleagues.

References:

Ibraghimov Orekhov V.Y., I., and m. Billeter, j. staff scientists at Biomol international. NMR, 2003, 27 (2) : p. 165-173
M Mayzel¹,K Kazimierczuk,V Yu Orekhov, Chem Commun (Camb). 2014 Aug 18th;50 (64) : 8947-50.
Doi: 10.1039 / c4cc03047h.
Krzysztof Kazimierczuk¹,Vladislav Yu Orekhov, Angew Chem Int Ed Engl. 2011 Jun 6;50 (24) : 5556-9.
Doi: 10.1002 / anie. 201100370.

Obtain the TopSpin NUS professional processing package